EDTA for Corneal Chelation

a.k.a. disodium ethylenediaminetetraacetic acid, N2EDTA chelation agent for removing corneal calcium deposits

Preparation method 1

  1. 0.3 ml sterile water injected into a lavender top, 3.0 ml K2-EDTA BD Vacutainer CBC tube (each tube has 5.4 mg K2-EDTA)
  2. Swirl the liquid around to dissolve the coating in the container
  3. Poor the contents into a second lavender top tube (will have to remove the tops of both tubes), repeat step 2
  4. Repeat step 3 for a total of 5 tubes
  5. results in 65 mg/ml of K2-EDTA, pH 5.8,
  6. Video example

Peparation method 2

  1. 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA)
  2. Swirl the liquid around to dissolve the coating of the container
  3. results in about 35 mg/ml K2-EDTA

Preparation method 3

  1. 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA)
  2. Use a sterile cotton tip applicator to rub off all of the EDTA into the liquid
  3. Use the cotton tip applicator to transfer the EDTA to the cornea
  4. results in about 52 mg/ml K2-EDTA
  5. Video Example

References

  1. "Off-the-Shelf" K2-EDTA for Calcific Band Keratopahy. Lee et al. Cornea 2018;37(7):916-918
  2. Simplified, Readily Available Method for the Treatment of Band Keratopathy With Ethylenediaminetetraacetic Acid. Narvaez J. et al. Cornea 2021;40(10):1360-1362